Eukaryotic genome annotation: a sample report

A snail-plot quality report from a eukaryotic genome assembly engagement, paired with completeness, scaffold statistics, and composition. The shape we hand off alongside the methods writeup.

About the deliverable

This is a one-page snail-plot report from a eukaryotic genome annotation engagement, a fungal assembly in this case. It pairs scaffold-level assembly statistics with completeness on a single shareable page, branded for the partner platform (1st BASE here, alongside Nova Lifetech).

It is the kind of one-pager a project lead can drop into a slide deck, and a reviewer can sanity-check in under a minute, without parsing log files.

Snail plot: scaffold statistics at a glance

The snail plot encodes the scaffold-size distribution as a logarithmic radial chart. Each ring marks an order of magnitude (1 kb, 10 kb, 100 kb), and the orange/blue colouring reflects whether scaffolds sit above or below the N50 threshold.

Headline numbers in the corner, total length 36.1 Mb, longest scaffold 498 kb, N50 60.8 kb, N90 8.6 kb, give the assembly contiguity at a glance. Composition (GC 50.4 %, AT 49.6 %, N 0 %) sits beside it for context.

Completeness

The completeness ring on the right summarises completeness against a lineage-appropriate reference set: 98.6 % complete, 1.4 % missing, 1.4 % duplicated, 0.4 % fragmented. For a fungal genome that completeness profile is in the 'ready for downstream annotation' range.

The duplication fraction is a flag worth investigating before publication, usually a sign of residual heterozygosity or unmerged haplotypes, and our delivery report calls it out explicitly so it does not get missed.

How this fits into a typical engagement

Every genomics engagement we run includes a sample-quality report like this one, alongside the methods writeup and a tab-separated annotation table. It is how the project lead and any external reviewer sign off on the assembly before downstream analysis begins.

Co-branded with the sequencing partner so it can drop straight into stakeholder communications. The same shape scales to bacterial, viral, and metagenome-assembled genomes, only the completeness reference set and composition expectations change.

↗Genomics, transcriptomics, and other technical services
↗AMR surveillance dashboard: a live project
About the deliverable
Snail plot: scaffold statistics at a glance
Completeness
How this fits into a typical engagement

Sequencing depth for microbiome studies: a tropical and SE Asia practical guide

How deep should you sequence each sample? The answer is sample-type dependent, and tropical and Southeast Asian samples typically need more depth than the canonical temperate references suggest. A practical guide with specific anchors for gut, soil, water, aquaculture, and fungal ITS work.

Wastewater AMR surveillance: methods and regulatory readiness for SE Asia utilities

Wastewater is becoming a recognised public-health signal for antimicrobial resistance. What samples, what methods, and how do you make a deliverable that holds up to a regulator? A guide for utilities, public-health agencies, and environmental-health researchers in Southeast Asia.

Tell us what you are working on.

A short note about your samples and questions is enough to start. We reply with how we would approach it.